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Avian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template.
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HyperScript Reverse Transcriptase is a novel reverse transcriptase engineered from M-MLV Reverse Transcriptase Compared to M-MLV Reverse Transcriptase HyperScript Reverse Transcriptase has significantly reduced RNase H activity and greatly enhanced thermostability It can withstand higher reaction temperatures and is particularly suitable for reverse transcription of RNA templates with complex secondary structures Furthermore HyperScript Reverse Transcriptase has enhanced affinity for templates making it especially suitable for reverse transcription of limiting amounts of template RNA or low-copy-number genes capable of generating cDNA products of up to 12 3 kb in length HyperScript Reverse Transcriptase is typically used for the synthesis of cDNA from RNA which can then be utilized for downstream qPCR assays or other experiments
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GenScript M-MuLV Reverse Transcriptase (M-MLV) is derived from a cloned region of the pol gene of MMLV and isolated from an E coli strain overexpressing this construct To increase cDNA yields and get a higher percentage of longer transcripts the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity and expressed free of exogenous RNases and other nucleases The enzyme can synthesize a complementary cDNA strand initiating from a primer using RNA as template (cDNA synthesis) making it ideal for a wide range of applications
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